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https://physoc.onlinelibrary.wiley.com/doi/full/10.1113/jphysiol.2011.207100
In vivo two-photon MNI-glutamate uncaging onto synapses while performing wholecell recording to measure resultant excitatory postsynaptic currents
Layer 2/3 pyramidal neurons in mouse visual cortex
Verifies (in vivo) that the functional strength of a spiny synapses is tightly correlated
to its head volume by directly stimulating individual spine synapses with glutamate and
measuring the resulting induced current.