Ju, N., Li, Y., Liu, F., Jiang, H., Macknik, S. L., Martinez-Conde, S., & Tang, S.
(2020). Spatiotemporal functional organization of excitatory synaptic inputs onto macaque V1
neurons. Nature communications, 11(1), 1-11. https://www.nature.com/articles/s41467-020-
14501-y
Two-photon dendritic imaging with a genetically-encoded glutamate sensor
(iGluSnFR) along with calcium imaging (RCaMP) in same neuron.
Macaque primary visual cortex
Authors use the glutamate sensor (iGluSnFR) expressed in a single visual cortex
cell to record its individual synaptic input’s receptive field properties (tuning to orientation,
spatial frequency, color). They were thus able to analyze how inputs tuned to different features
are spatially distributed relative to each other (i.e. clustering).