Kim, W. B., & Cho, J. H. (2017). Encoding of discriminative fear memory by inputspecific LTP in the amygdala. Neuron, 95(5), 1129-1146.
https://www.sciencedirect.com/science/article/pii/S0896627317306955
fos labeling, optogenetic stimulation, whole cell patch clamp electrophysiology of
AMPA/NMDA conductances (in slice)
ACx -> LA (did not find effect in MGN -> ACx)
This paper explores in detail the connectivity from ACx/MGN -> LA They first
robustly label 4kHz tone specific neurons in ACx/MGN and compare a later labeled 12kHz
subset getting an estimate of the number and specificity of cells representing the tones. They
then perform whole cell electrophysiology in LA to determine the number of cells that respond to
a specific tone (vs control where all ACx/MGN cells were optogentically activated).
“Using a combined approach of neural activity-dependent behavioral labeling
(Guenthner et al., 2013), optogenetic stimulations (Yizhar et al., 2011), and electrophysiological
recordings (Cho et al., 2013), we found that postsynaptically expressed LTP was induced
selectively in the CS-specific ACx/MGN-LA pathways after auditory discriminative fear
conditioning in mice, whereas LTP was not detected in randomly selected ACx/MGN-LA
pathways. Moreover, optogenetically induced depotentiation of the CS specific ACx/MGN-LA
pathways prevented the recall of fear memory for the auditory CS.”
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