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https://www.nature.com/articles/s41586-020-03134-2
Slice electrophysiology of synaptically connected pyramidal neurons with correlated
light and electron microscopy of all putative synaptic contacts between the recorded neurons
Mouse somatosensory cortex slice
Uses slice electrophysiology to measure functional connectivity between two
neurons and compares this to electron microscopically determined number and area of synaptic
contacts between same neurons. Finds a linear relationship between total cumulative PSD area
and strength.
“We find a linear relationship between synapse size and strength, providing the missing
link in assigning physiological weights to synapses reconstructed from electron microscopy.”